Can i autoclave hcl

Tris-Cl 1 M Dissolve Adjust the volume of the solution to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving. If the 1 M solution has a yellow color, discard it and obtain Tris of better quality. For example, a 0. Adjust the volume to 3 liters with H2O. Dispense into aliquots. Sterilize by autoclaving. The final concentrations of the ingredients are 3.

Store the solution at 4 C and transfer it to an ice bucket just before use. Measure Mix the solution well and then sterilize by passing it through a 0. Store the sterile freezing medium at a controlled room temperature C. EDTA 0. Stir vigorously on a magnetic stirrer. If I am wrong so plz tell me Proteins and thermo-sensitive components must be added after autoclaving the medium, but must be sterilized by filtration with a 0.

Afterall you will autoclave your media that will do. Don't worry Actually Media contain various amino acids and co-factors which will degrade when undergoes autoclaving. I used PI stain for Jurkat cell line, in this control normal cells also give fluorescence.

Can anyone suggest what can be the problem? What is the procedure to submit raw data of microarrays? In which microarray database I should submit my raw data and is it compulsory to submit the data before communicate the article?

We have an IMR cell line. Can anyone suggest whether this is a good cell line for the above study? I want to do mitochondrial membrane potential of human cell line. I would like to perform fluorescence microscopy on a HL cell line using Hoechst.

How to stain the suspension culture with this stain? Can anybody send me a detailed protocol? In this kit it is given to sonicate or homogenize the cells but we don't have sonicator in our lab I am doing this assay on HL cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei.

Does anyone have experience with this assay? Please send me any When I tried to energy minimization my system, I got fatal error as below. Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis.

For now, I am able to use the Translate command in PyMol There appears to be a core problem for I am going to have 3 different probes in my qPCR work that I am going to do. Andrii Tarieiev In case that precise concentration of HCl is very important for you, I recommend you sterilization through filtering, because the concentration concentration may be reduced during the procedure of autoclavation. Abdelmoumen Taoutaou You don't need to autoclave HCl, you'll autoclave the culture medium after pH adjustment.

Abhirama Krishna as mentioned above, you can use double distilled water to prepare culture medium, and use filters to sterilize the HCl prepared with DD water , anyway you are going to autoclave the medium after PH adjustment, , ,. Christian Praetorius Autoclaving HCl will decrease the concentration since HCl will get into the gas phase when heated.

Essam Kotb no need to autoclave just add it to the medium after autoclaving it under aseptic conditions. Deepa Gandhi dear friends as per my the knowledge we can not autoclave cell culture medium. Deepa Gandhi Essam Kotb Steingrimur Stefansson Simple bacterial media can be autoclaved. You do not autoclave tissue culture media.